chromatography – a broad range of physical methods used to separate and or to analyze complex mixtures. The components to be separated are distributed between two phases: a stationary phase bed and a mobile phase which percolates through the stationary bed.
- chromatography can purify basically any soluble or volatile substance if the right adsorbent material, carrier fluid, and operating conditions are employed.
- chromatography can be used to separate delicate products since the conditions under which it is performed are not typically severe.
- chromatography is well suited to a variety of uses in the field of biotechnology, such as separating mixtures of proteins.
Chromatography – laboratory techniques for the separation of mixtures. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate. The separation is based on differential partitioning between the mobile and stationary phases. Differences in a compound’s partition coefficient result in differential retention on the stationary phase and thus changing the separation.
Chromatography may be preparative or analytical.
- preparative chromatography is to separate the components of a mixture for more advanced use (and is thus a form of purification).
- analytical chromatography is done normally with smaller amounts of material and is for measuring the relative proportions of analytes in a mixture.
- soluble – capable or susceptible of being liquefied or emulsified
- volatile – readily becoming fine particles of matter floating in the air at a relatively low temperature; liable to sudden changes